HB-EGF
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | XP_023257950.1 |
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Species | Seriola |
Sequence | Ser88-Leu179 |
Purity | ≥ 90% as analyzed by SDS-PAGE |
Endotoxin Level | < 0.2 EU/ µg of protein by gel clotting method |
Biological Activity | ED50 < 1.0 µg/ml, measured by a cell proliferation assay using BALB/3T3 cells, corresponding to a specific activity of > 1.0 × 103 units/mg. |
Expression System | E.coli |
Theoretical Molecular Weight | 10.06 kDa |
Formulation | Lyophilized from a 0.2 µm filtered solution in 50 mM Tris, 150 mM Nacl, pH 8.0 |
Reconstitution | Before opening, centrifuge the vial briefly to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O up to 100 µg/ml |
Storage & Stability | Upon receiving, this product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable up to 1 week at 4 °C or up to 3 months at -20 °C. Avoid repeated freeze-thaw cycles. |
Target Background | HB-EGF-like growth factor is synthesized as a membrane-anchored mitogenic and chemotactic glycoprotein. An epidermal growth factor produced by monocytes and macrophages, due to an affinity for heparin is termed HB-EGF. It has been shown to play a role in wound healing, cardiac hypertrophy, and heart development and function. |
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