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PDGF-BB

     
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Product Information
Primary Accession B1H0W5
Species Bovine
Sequence Ser82-Thr190
Purity ≥ 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.1 EU/ µg of protein by gel clotting method
Biological Activity ED50 < 20.00 ng/ml as determined by a dose-response proliferation assay using murine Balb/c 3T3 cells. Based on the ED50, the calculated specific activity is approximately > 0.5 × 105 IU/mg. It is recommended to experimentally determine the optimal concentration for each specific application by performing a dose response assay.
Expression System P. pastoris
Theoretical Molecular Weight 12 kDa
Formulation Lyophilized from a 0.2 µm filtered solution in 20 mM NaAc, pH 5.0.
Reconstitution Before opening, centrifuge the vial briefly to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O up to 100 µg/ml.
Storage & Stability Upon receiving, the lyophilized product remains stable for up to 6 months at lower than -70 °C. Upon reconstitution, the product is stable for up to 1 week at 4 °C or up to 3 months at -20 °C. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is storage at -20 °C.
Additional Information
Target Background Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in an animal study by Lynch and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three subforms: PDGF-AA, PDGF-BB, and PDGF-AB. It is involved in many biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is mediated by two receptors (PDGFR-α and PDGFR-β).
Protein Information
Research Areas
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Cat# PVGS1685
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