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CCL25

     
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Product Information
Primary Accession O35903
Species Mouse
Sequence Gln24-Asn144
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
Endotoxin Level < 1 EU/ µg of protein by LAL method
Biological Activity The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 5.0-50.0 ng/ml.
Expression System E. coli
Theoretical Molecular Weight 14.1 kDa
Formulation Lyophilized from a 0.2 µm filtered solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at -20°C or -70°C. Upon reconstitution, the product should be stable for up to 1 week at 2-8°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles.
Additional Information
Gene ID 20300
Other Names C-C motif chemokine 25, Chemokine TECK, Small-inducible cytokine A25, Thymus-expressed chemokine, Ccl25, Scya25, Teck
Target Background CCL25 is a new member of the CC family chemokine. It is also called Thymus-expressed chemokine (TECK) because it is restricted produced by thymus and intestine. Especially, the dendritic cells derived from thymus but not bone marrow had been identified to be the source of CCL25. By binding with CCR9, it elicits its effects of chemotactic for thymocytes, macrophages, and dendritic cells. Additionally, CCL25 takes part in regulating the development of T-cells.
Protein Information
Name Ccl25
Synonyms Scya25, Teck
Function Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
Cellular Location Secreted.
Tissue Location Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine
Research Areas
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$ 0.00
Cat# PVGS1660
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