DCI Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P42126 |
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Clone Names | 90921080 |
Gene ID | 1632 |
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Other Names | Enoyl-CoA delta isomerase 1, mitochondrial, 2-trans-enoyl-CoA isomerase, Delta(3), Delta(2)-enoyl-CoA isomerase, D3, D2-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase, ECI1, DCI |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP9230b was selected from the C-term region of human DCI. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | ECI1 |
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Synonyms | DCI |
Function | Key enzyme of fatty acid beta-oxidation (Probable). Able to isomerize both 3-cis (3Z) and 3-trans (3E) double bonds into the 2- trans (2E) form in a range of enoyl-CoA species, with a preference for (3Z)-enoyl-CoAs over (3E)-enoyl-CoAs (By similarity) (PubMed:7818490). The catalytic efficiency of this enzyme is not affected by the fatty acyl chain length (By similarity). |
Cellular Location | Mitochondrion matrix {ECO:0000250|UniProtKB:P23965} |
Tissue Location | Expressed in liver (at protein level). |
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Provided below are standard protocols that you may find useful for product applications.
Background
DCI encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates.
References
Partanen,S.T., et.al., J. Mol. Biol. 342 (4), 1197-1208 (2004)Wistow,G., et.al, Mol. Vis. 8, 171-184 (2002)
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