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PRP4 Antibody (N-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q13523 |
|---|---|
| Clone Names | 3012011 |
| Gene ID | 8899 |
|---|---|
| Other Names | Serine/threonine-protein kinase PRP4 homolog, PRP4 kinase, PRP4 pre-mRNA-processing factor 4 homolog, PRPF4B, KIAA0536, PRP4, PRP4H, PRP4K |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP7551a was selected from the N-term region of human PRP4 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | PRP4K (HGNC:17346) |
|---|---|
| Function | Serine/threonine kinase involved in spliceosomal assembly as well as mitosis and signaling regulation (PubMed:10799319, PubMed:12077342, PubMed:17513757, PubMed:17998396). Connects chromatin mediated regulation of transcription and pre-mRNA splicing (PubMed:12077342). During spliceosomal assembly, interacts with and phosphorylates PRPF6 and PRPF31, components of the U4/U6-U5 tri-small nuclear ribonucleoprotein (snRNP), to facilitate the formation of the spliceosome B complex. Plays a role in regulating transcription and the spindle assembly checkpoint (SAC) (PubMed:20118938). Associates with U5 snRNP and NCOR1 deacetylase complexes which may allow a coordination of pre-mRNA splicing with chromatin remodeling events involved in transcriptional regulation (PubMed:12077342). Associates and probably phosphorylates SMARCA4 and NCOR1 (PubMed:12077342). Phosphorylates SRSF1 (PubMed:11418604). Associates with kinetochores during mitosis and is necessary for recruitment and maintenance of the checkpoint proteins such as MAD1L1 and MAD12L1 at the kinetochores (PubMed:17998396). Phosphorylates and regulates the activity of the transcription factors such as ELK1 and KLF13 (PubMed:10799319, PubMed:17513757). Phosphorylates nuclear YAP1 and WWTR1/TAZ which induces nuclear exclusion and regulates Hippo signaling pathway, involved in tissue growth control (PubMed:29695716). |
| Cellular Location | Nucleus. Chromosome, centromere, kinetochore Note=Located throughout the nucleus, excluding the nucleolus but enriched in multiple speckles. |
| Tissue Location | Ubiquitous. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Pre-mRNA splicing occurs in two sequential transesterification steps, and PRP4 is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs).
References
Dellaire, G., et al., Mol. Cell. Biol. 22(14):5141-5156 (2002).Kojima, T., et al., J. Biol. Chem. 276(34):32247-32256 (2001).Huang, Y., et al., Biochem. Biophys. Res. Commun. 271(2):456-463 (2000).Gross, T., et al., Nucleic Acids Res. 25(5):1028-1035 (1997).
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