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SSB3 Antibody (Center) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q6PJ21 |
|---|---|
| Clone Names | 80924219 |
| Gene ID | 90864 |
|---|---|
| Other Names | SPRY domain-containing SOCS box protein 3, SSB-3, SPSB3, C16orf31, SSB3 |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP6691c was selected from the Center region of human SSB3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | SPSB3 {ECO:0000303|PubMed:38418882, ECO:0000312|HGNC:HGNC:30629} |
|---|---|
| Function | Substrate-recognition component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex, also named CRL5 complex), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as CGAS and SNAI1 (PubMed:29059170, PubMed:38418882). The ECS(SPSB3) complex catalyzes 'Lys-48'-linked ubiquitination of nuclear CGAS in cycling cells, leading to its degradation (PubMed:38418882). Recognizes and binds nucleosome-bound CGAS: ubiquitination and degradation of nuclear CGAS during G1 and G2 phases is required to promote low intranuclear CGAS abundance before the next mitotic cycle (PubMed:38418882). The ECS(SPSB3) complex also mediates ubiquitination and degradation of phosphorylated SNAI1 (PubMed:29059170). |
| Cellular Location | Nucleus. Note=Nuclear SPSB3 levels increase during interphase |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
SSB3 may be a substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
References
Kile,B.T., Trends Biochem. Sci. 27 (5), 235-241 (2002)
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