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Zebrafish hey2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Zebrafish hey2 Antibody (C-term) Azb21564b
    Anti-Zebrafish hey2 Antibody (C-term)at 1:2000 dilution + ZF4 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9I9L0
Reactivity Zebrafish
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Clone/Animal Names RB53204
Calculated MW 34329 Da
Antigen Region 224-260 aa
Additional Information
Gene ID 58146
Other Names Hairy/enhancer-of-split related with YRPW motif protein 2, Protein gridlock, hey2, grl
Target/Specificity This Zebrafish hey2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 224-260 amino acids from the C-terminal region of Zebrafish hey2.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZebrafish hey2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name hey2
Synonyms grl
Function Transcriptional repressor (By similarity). Downstream effector of Notch signaling which regulates cell fate choice in angioblasts. Represses the venous cell fate, thereby promoting the arterial cell fate and aorta formation.
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00380, ECO:0000255|PROSITE-ProRule:PRU00981}
Research Areas
Citations (0)
citation

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Background

Transcriptional repressor (By similarity). Downstream effector of Notch signaling which regulates cell fate choice in angioblasts. Represses the venous cell fate, thereby promoting the arterial cell fate and aorta formation.

References

Zhong T.P.,et al.Science 287:1820-1824(2000).
Zhong T.P.,et al.Nature 414:216-220(2001).
Winkler C.,et al.Dev. Genes Evol. 213:541-553(2003).

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$ 365.00
$ 140.00
Cat# Azb21564b
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