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DANRE hnf1bb Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - DANRE hnf1bb Antibody (Center) Azb18694a
    Western blot analysis of lysate from zebra fish muscle tissue lysate, using DANRE hnf1bb Antibody (Center)(Cat. #Azb18694a). Azb18694a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8UW00
Reactivity Zebrafish
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Clone/Animal Names RB48027
Calculated MW 58320 Da
Antigen Region 212-245 aa
Additional Information
Gene ID 324283
Other Names Hepatocyte nuclear factor 1-beta-B, hnf1bb {ECO:0000312|ZFIN:ZDB-GENE-030131-3003}
Target/Specificity This DANRE hnf1bb antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 212-245 amino acids from the Central region of DANRE hnf1bb.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDANRE hnf1bb Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name hnf1bb {ECO:0000312|ZFIN:ZDB-GENE-030131-3003}
Function Transcription factor that binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (By similarity). Acts downstream of hnf1ba but is not required for induction of rhombomere r5/r6 gene expression in the hindbrain.
Cellular Location Nucleus {ECO:0000250|UniProtKB:P35680, ECO:0000255|PROSITE-ProRule:PRU00108}
Tissue Location First expressed at stage 10 in the intermediate mesoderm. Expressed in rhombomere r5 by 14 hpf with expression diminishing by 18 hpf.
Research Areas
Citations (0)
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Background

Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (By similarity). Acts downstream of hnf1ba but is not required for induction of rhombomere r5/r6 gene expression in the hindbrain.

References

Gong H.-Y.,et al.Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
Choe S.-K.,et al.Zebrafish 5:179-187(2008).

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$ 365.00
$ 140.00
Cat# Azb18694a
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