DANRE hnf1bb Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q8UW00 |
Reactivity | Zebrafish |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Clone/Animal Names | RB48027 |
Calculated MW | 58320 Da |
Antigen Region | 212-245 aa |
Gene ID | 324283 |
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Other Names | Hepatocyte nuclear factor 1-beta-B, hnf1bb {ECO:0000312|ZFIN:ZDB-GENE-030131-3003} |
Target/Specificity | This DANRE hnf1bb antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 212-245 amino acids from the Central region of DANRE hnf1bb. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | DANRE hnf1bb Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | hnf1bb {ECO:0000312|ZFIN:ZDB-GENE-030131-3003} |
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Function | Transcription factor that binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (By similarity). Acts downstream of hnf1ba but is not required for induction of rhombomere r5/r6 gene expression in the hindbrain. |
Cellular Location | Nucleus {ECO:0000250|UniProtKB:P35680, ECO:0000255|PROSITE-ProRule:PRU00108} |
Tissue Location | First expressed at stage 10 in the intermediate mesoderm. Expressed in rhombomere r5 by 14 hpf with expression diminishing by 18 hpf. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (By similarity). Acts downstream of hnf1ba but is not required for induction of rhombomere r5/r6 gene expression in the hindbrain.
References
Gong H.-Y.,et al.Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
Choe S.-K.,et al.Zebrafish 5:179-187(2008).
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