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DANRE pou3f3a Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - DANRE pou3f3a Antibody (C-term) Azb10007b
    DANRE pou3f3a Antibody (C-term) (Cat. #Azb10007b) western blot analysis in zebra fish brain tissue lysates (35ug/lane).This demonstrates the DANRE pou3f3a antibody detected the DANRE pou3f3a protein (arrow).
    detail
  • WB - DANRE pou3f3a Antibody (C-term) Azb10007b
    Anti-DANRE pou3f3a Antibody (C-term) at 1:1000 dilution + Zebrafish lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P56224
Reactivity Human, Zebrafish
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 47216 Da
Antigen Region 381-407 aa
Additional Information
Gene ID 30386
Other Names POU domain, class 3, transcription factor 3-A, Brain-specific homeobox/POU domain protein 11, Brain-11, zfBrn-11, Class III POU domain protein taichi, POU domain protein 12, ZP-12, pou3f3a, brn-11, brn11, pou12, tai-ji, zp12, zp12pou
Target/Specificity This DANRE pou3f3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 381-407 amino acids from the C-terminal region of DANRE pou3f3a.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDANRE pou3f3a Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name pou3f3a
Synonyms brn-1.1, brn1.1, pou12, tai-ji, zp12, zp
Function Transcription factor that may play important roles in patterning the embryonic brain.
Cellular Location Nucleus.
Tissue Location Predominantly expressed in the embryonic and adult central nervous system. In adults, isoform 2 is expressed in the brain, ovary, basal cells of the skin and muscle satellite cells
Research Areas
Citations (0)
citation

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Background

Transcription factor that may play important roles in patterning the embryonic brain.

References

Spaniol P., et al. Nucleic Acids Res. 24:4874-4881(1996).
Lim J.H., et al. Biochim. Biophys. Acta 1397:253-256(1998).
Huang S., et al. Mech. Dev. 71:23-35(1998).
Shah D., et al. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
Sampath K., et al. Biochem. Biophys. Res. Commun. 219:565-571(1996).

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$ 365.00
$ 140.00
Cat# Azb10007b
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