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FLNA Antibody [Knockout Validated]
Mouse Monoclonal Antibody (Mab) KO
![IF - FLNA Antibody [Knockout Validated] AW5707-U100](/assets/uploads/products/201801/AM2240B_IF_1.jpg)
![WB - FLNA Antibody [Knockout Validated] AW5707-U100](/assets/uploads/products/201601/AM2240B_WB_1.jpg)
![WB - FLNA Antibody [Knockout Validated] AW5707-U100](/assets/uploads/products/201311/AW5707_WB_1.jpg)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF |
|---|---|
| Primary Accession | P21333 |
| Reactivity | Human, Mouse, Rat |
| Host | Mouse |
| Clonality | Monoclonal |
| Calculated MW | 280739 Da |
| Isotype | IgG1 |
| Antigen Source | Human |
| Gene ID | 2316 |
|---|---|
| Antigen Region | 1-360 aa |
| Other Names | Filamin-A, FLN-A, Actin-binding protein 280, ABP-280, Alpha-filamin, Endothelial actin-binding protein, Filamin-1, Non-muscle filamin, FLNA, FLN, FLN1 |
| Dilution | IF~~1:25 WB~~1:500-1:2000 |
| Target/Specificity | Purified His-tagged FLNA protein was used to produced this monoclonal antibody. |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | FLNA Antibody [Knockout Validated] is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | FLNA |
|---|---|
| Synonyms | FLN, FLN1 |
| Function | Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNB may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface- localized furin, modulates its rate of internalization and directs its intracellular trafficking (By similarity). Involved in ciliogenesis. Plays a role in cell-cell contacts and adherens junctions during the development of blood vessels, heart and brain organs. Plays a role in platelets morphology through interaction with SYK that regulates ITAM- and ITAM-like-containing receptor signaling, resulting in by platelet cytoskeleton organization maintenance (By similarity). During the axon guidance process, required for growth cone collapse induced by SEMA3A-mediated stimulation of neurons (PubMed:25358863). |
| Cellular Location | Cytoplasm, cell cortex. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q8BTM8}. Perikaryon {ECO:0000250|UniProtKB:Q8BTM8}. Cell projection, growth cone {ECO:0000250|UniProtKB:Q8BTM8}. Cell projection, podosome {ECO:0000250|UniProtKB:Q8BTM8}. Note=Colocalizes with CPMR1 in the central region of DRG neuron growth cone (By similarity). Following SEMA3A stimulation of DRG neurons, colocalizes with F-actin (By similarity). Localized to the core of myotube podosomes (By similarity). {ECO:0000250|UniProtKB:Q8BTM8} |
| Tissue Location | Ubiquitous. |
Provided below are standard protocols that you may find useful for product applications.
Background
Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking (By similarity). Involved in ciliogenesis.
References
Gorlin J.B., et al. J. Cell Biol. 111:1089-1105(1990).
Patrosso M.C., et al. Genomics 21:71-76(1994).
Chen E.Y., et al. Hum. Mol. Genet. 5:659-668(1996).
Li J., et al. Mol. Cell. Proteomics 9:2517-2528(2010).
Ota T., et al. Nat. Genet. 36:40-45(2004).
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