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His Tag Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - His Tag Antibody AW5661-U100
    All lanes : Anti-6×His Tag Antibody at 1:1000 dilution (upper) or GAPDH (lower) Lane 1: 293T/17 transfected with 12tag lysate (10ug) Lane 2: Non-transfected 293T/17 lysate (10ug) Lane 3: 12tag recombinant protein lysate (1ug) Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45-50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Host Rabbit
Clonality Polyclonal
Calculated MW 45-50kd
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Antigen Region NA aa
Other Names Poly-His, Hexa-HIS, Hexa-Histidine
Dilution WB~~1:1000
Target/Specificity Poly-HIS peptide were used to produced this antibody.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHis Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)
citation

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Background

Epitope tags consisting of short sequences recognized by well-characterized monoclonal or polyclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal or polyclonal antibody provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS polyclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins.

References

Hochuli E, Doebeli H, and Schacher A. New metal chelate absorbent selective for proteins and peptides containing neighboring histidine residues. J. Chromatogr. 1987;411:177-184.

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$ 370.00
Cat# AW5661-U100
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