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FAM120A Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • IF - FAM120A Antibody AW5641-U100
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with AW5641 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
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  • WB - FAM120A Antibody AW5641-U100
    All lanes : Anti-FAM120A Antibody at1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: U-2OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 122 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • IHC-P - FAM120A Antibody AW5641-U100
    AW5641 staining FAM120A in human brain sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IF, IHC-P, WB
Primary Accession Q9NZB2
Reactivity Human
Host Mouse
Clonality Monoclonal
Calculated MW H=122,69,117,72,125;M=122 KDa
Isotype IgG1,k
Antigen Source HUMAN
Additional Information
Gene ID 23196
Antigen Region 280-600 aa
Other Names Constitutive coactivator of PPAR-gamma-like protein 1, Oxidative stress-associated Src activator, Protein FAM120A, FAM120A, C9orf10, KIAA0183, OSSA
Dilution IF~~1:25
WB~~1:2000
IHC-P~~1:25
Target/Specificity This FAM120A antibody is generated from a mouse immunized with a recombinant protein of human FAM120A.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFAM120A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FAM120A
Function Component of the oxidative stress-induced survival signaling. May regulate the activation of SRC family protein kinases (PubMed:19015244). May act as a scaffolding protein enabling SRC family protein kinases to phosphorylate and activate PI3-kinase (PubMed:19015244). Binds IGF2 RNA and promotes the production of IGF2 protein (PubMed:19015244).
Cellular Location Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Translocates from the cytosol to plasma membrane after UV irradiation.
Tissue Location Widely expressed (PubMed:14585507). In gastric mucosa, detected in the bottom region of the foveolar epithelium (at protein level) (PubMed:19015244).
Research Areas
Citations (0)
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Background

May participate in mRNA transport in the cytoplasm (By similarity). Critical component of the oxidative stress-induced survival signaling. Activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. Binds RNA and promotes the secretion of IGF-II. May play a pivotal role in the progression of scirrhous- type gastric cancer by supporting cancer cell survival in environments with various oxidative stresses.

References

Brahmbhatt S.B.,et al.Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
Humphray S.J.,et al.Nature 429:369-374(2004).
Nagase T.,et al.DNA Res. 3:17-24(1996).
Ohara O.,et al.Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
Holden S.,et al.Gene 318:149-161(2003).

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$ 380.00
Cat# AW5641-U100
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