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Mlkl Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| WB |
|---|---|
| Primary Accession | Q9D2Y4 |
| Reactivity | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | M=54,53 KDa |
| Isotype | Rabbit IgG |
| Antigen Source | HUMAN |
| Gene ID | 74568 |
|---|---|
| Antigen Region | 444-472 aa |
| Other Names | Mixed lineage kinase domain-like protein, Mlkl {ECO:0000312|EMBL:AAH237551, ECO:0000312|MGI:MGI:1921818} |
| Dilution | WB~~0.25 |
| Target/Specificity | This Mouse Mlkl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-472 amino acids from the C-terminal region of mouse Mlkl. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mlkl Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Mlkl {ECO:0000303|PubMed:23835476, ECO:0000312|MGI:MGI:1921818} |
|---|---|
| Function | Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process (PubMed:23835476, PubMed:24012422, PubMed:24019532, PubMed:27321907, PubMed:32200799, PubMed:32296175). Does not have protein kinase activity (PubMed:24012422). Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed:23835476, PubMed:24012422, PubMed:24019532, PubMed:27321907). In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol (PubMed:32200799, PubMed:32296175). Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function (By similarity). |
| Cellular Location | Cytoplasm. Cell membrane. Nucleus. Note=Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction (By similarity). Localizes to the nucleus in response to orthomyxoviruses infection (PubMed:32200799). {ECO:0000250|UniProtKB:Q8NB16, ECO:0000269|PubMed:32200799} |
| Tissue Location | Highly expressed in thymus, colon, intestine, liver, spleen and lung. Expressed at much lower level in skeletal muscle, heart and kidney. Not detected in brain |
Research Areas
Citations (0)
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
The protein kinase domain is predicted to be catalytically inactive. Molecular function: protein binding. There are two isoforms.
References
Bisson, N., et al. Cell Cycle 7(7):909-916(2008)
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$ 370.00
Cat# AW5609-U100
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