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PPM1H Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - PPM1H Antibody (Center) AW5604-U100
    All lanes : Anti-PPM1H Antibody (Center) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: mouse spleen lysate Lane 4: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 56 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - PPM1H Antibody (Center) AW5604-U100
    Formalin-fixed and paraffin-embedded human brain tissue reacted with PPM1H Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - PPM1H Antibody (Center) AW5604-U100
    PPM1H Antibody (Center)(Cat. #AW5604) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB
Primary Accession Q9ULR3
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=56;M=56,52;R=56 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 57460
Antigen Region 235-263 aa
Other Names Protein phosphatase 1H, PPM1H, ARHCL1, KIAA1157, URCC2
Dilution WB~~1:2000
IHC-P~~1:50~100
FC~~1:10~50
Target/Specificity This PPM1H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-263 amino acids from the Central region of human PPM1H.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPM1H Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPM1H
Synonyms ARHCL1, KIAA1157, URCC2
Function Dephosphorylates CDKN1B at 'Thr-187', thus removing a signal for proteasomal degradation.
Cellular Location Nucleus. Cytoplasm
Citations (0)
citation

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References

Sugiura,T., et.al., Cancer Biol. Ther. 7 (2), 285-292 (2008)
Labes,M., et.al., Mol. Cell. Neurosci. 12 (1-2), 29-47 (1998)

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$ 370.00
Cat# AW5604-U100
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