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MAML2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - MAML2 Antibody (Center) AW5597-U100
    All lanes : Anti-MAML2 Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 125 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC - MAML2 Antibody (Center) AW5597-U100
    AW5597 staining MAML2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q8IZL2
Other Accession A5D7F6
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW H=125 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 84441
Antigen Region 780-814 aa
Other Names Mastermind-like protein 2, Mam-2, MAML2, KIAA1819
Dilution WB~~1:2000
IHC~~1:25
Target/Specificity This MAML2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 780-814 amino acids from the Central region of human MAML2.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAML2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAML2
Synonyms KIAA1819
Function Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Potentiates activation by NOTCH3 and NOTCH4 more efficiently than MAML1 or MAML3.
Cellular Location Nucleus speckle. Note=Nuclear, in a punctate manner
Tissue Location Widely expressed with high levels detected in placenta, salivary gland and skeletal muscle
Research Areas
Citations (0)
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Background

Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Potentiates activation by NOTCH3 and NOTCH4 more efficiently than MAML1 or MAML3.

References

Tonon G.,et al.Nat. Genet. 33:208-213(2003).
Tonon G.,et al.Nat. Genet. 33:408-408(2003).
Nagase T.,et al.DNA Res. 8:85-95(2001).
Taylor T.D.,et al.Nature 440:497-500(2006).
Enlund F.,et al.Exp. Cell Res. 292:21-28(2004).

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$ 370.00
Cat# AW5597-U100
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