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PEF1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q9UBV8 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | H=30;M=29;R=30 KDa |
| Isotype | Rabbit IgG |
| Antigen Source | HUMAN |
| Gene ID | 553115 |
|---|---|
| Antigen Region | 95-121 aa |
| Other Names | Peflin, PEF protein with a long N-terminal hydrophobic domain, Penta-EF hand domain-containing protein 1, PEF1, ABP32 |
| Dilution | WB~~1:1000 |
| Target/Specificity | This PEF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-121 amino acids from the Central region of human PEF1. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PEF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PEF1 (HGNC:30009) |
|---|---|
| Synonyms | ABP32 |
| Function | Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as a calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats (PubMed:27716508). In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification (PubMed:27716508). Its role in the heterodimer formed with PDCD6 is however unclear: some evidence shows that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium (PubMed:27716508). Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6 (PubMed:11278427). Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31 (By similarity). |
| Cellular Location | Cytoplasm. Endoplasmic reticulum {ECO:0000250|UniProtKB:Q641Z8}. Membrane; Peripheral membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Peripheral membrane protein. Note=Membrane-associated in the presence of Ca(2+) (PubMed:11278427). Localizes to endoplasmic reticulum exit site (ERES) (By similarity). {ECO:0000250|UniProtKB:Q641Z8, ECO:0000269|PubMed:11278427} |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. The encoded protein has been shown to form a heterodimer with the programmed cell death 6 gene product and may modulate its function in Ca(2+) signaling. Alternative splicing results in multiple transcript variants and a pseudogene has been identified on chromosome 1.
References
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Hansen, C., et al. FEBS Lett. 545 (2-3), 151-154 (2003) :
Satoh, H., et al. Biochim. Biophys. Acta 1600 (1-2), 61-67 (2002) :
Kitaura, Y., et al. Arch. Biochem. Biophys. 399(1):12-18(2002)
Kitaura, Y., et al. J. Biol. Chem. 276(17):14053-14058(2001)
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