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CD74 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • IHC - CD74 Antibody AW5503-U100
    Immunohistochemical analysis of paraffin-embedded Human tonsil section using Pink1(Cat#AW5503). AW5503 was diluted at 1:400 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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  • WB - CD74 Antibody AW5503-U100
    All lanes : Anti-CD74 Antibody at 1:1000 dilution Lane 1: Daudi whole cell lysates Lane 2: Raji whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG (H+L),Peroxidase conjugated at 1/5,000 dilution Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • FC - CD74 Antibody AW5503-U100
    Flow cytometric analysis of Raji cells using CD74(green, Cat#AW5503) compared to an isotype control of mouse IgG2b(blue). AW5502 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P04233
Reactivity Human
Host Mouse
Clonality Monoclonal
Calculated MW H=34,26,18 KDa
Isotype IgG2b,κ
Antigen Source HUMAN
Additional Information
Gene ID 972
Antigen Region 1-232 aa
Other Names HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, Ii, p33, CD74, CD74, DHLAG
Dilution IHC~~1:400
WB~~1:1000
FC~~1:25
Target/Specificity This antibody is generated from a mouse immunized with a recombinant protein.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD74 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD74 (HGNC:1697)
Synonyms DHLAG
Function Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. [Isoform p41]: Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs). Has antiviral activity by stymieing the endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2 (PubMed:32855215). Disrupts cathepsin-mediated Ebola virus glycoprotein processing, which prevents viral fusion and entry. This antiviral activity is specific to p41 isoform (PubMed:32855215).
Cellular Location Cell membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane. Golgi apparatus, trans-Golgi network. Endosome. Lysosome. Secreted. Note=Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane
Tissue Location Detected in urine (at protein level). [Isoform p33]: In B cells, represents 70% of total CD74 expression.
Research Areas
Citations (0)
citation

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Background

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.

References

Claesson L.,et al.Proc. Natl. Acad. Sci. U.S.A. 80:7395-7399(1983).
Strubin M.,et al.EMBO J. 3:869-872(1984).
Kudo J.,et al.Nucleic Acids Res. 13:8827-8841(1985).
O'Sullivan D.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:4484-4488(1986).
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.

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$ 380.00
Cat# AW5503-U100
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