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UBE2L3 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P |
|---|---|
| Primary Accession | P68036 |
| Other Accession | P68037, Q3MHP1, NP_003338 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | H=18,14;M=18 KDa |
| Isotype | Rabbit IgG |
| Antigen Source | HUMAN |
| Gene ID | 7332 |
|---|---|
| Antigen Region | 123-153 aa |
| Other Names | Ubiquitin-conjugating enzyme E2 L3, L-UBC, UbcH7, Ubiquitin carrier protein L3, Ubiquitin-conjugating enzyme E2-F1, Ubiquitin-protein ligase L3, UBE2L3, UBCE7, UBCH7 |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Target/Specificity | This UBE2L3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-153 amino acids from the C-terminal region of human UBE2L3. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | UBE2L3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | UBE2L3 |
|---|---|
| Synonyms | UBCE7, UBCH7 |
| Function | Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN, RNF31 and ARIH1, that function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down- regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis. |
| Cellular Location | Nucleus. Cytoplasm |
| Tissue Location | Ubiquitous, with highest expression in testis. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2L3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro.
References
Moynihan, T.P., et al., Genomics 51(1):124-127 (1998).
Moynihan, T.P., et al., Mamm. Genome 7(7):520-525 (1996).
Nuber, U., et al., J. Biol. Chem. 271(5):2795-2800 (1996).
Robinson, P.A., et al., Mamm. Genome 6(10):725-731 (1995).
Ardley, H.C., et al., Biochim. Biophys. Acta 1491 (1-3), 57-64 (2000).
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