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AVPR2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - AVPR2 Antibody (C-term) AW5433-U100
    All lanes : Anti-AVPR2 Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysates Lane 2: Jurkat whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • FC - AVPR2 Antibody (C-term) AW5433-U100
    Overlay histogram showing Jurkat cells stained with AW5433 (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody ( , 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P30518
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=40 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 554
Antigen Region 343-377 aa
Other Names Vasopressin V2 receptor, V2R, AVPR V2, Antidiuretic hormone receptor, Renal-type arginine vasopressin receptor, AVPR2, ADHR, DIR, DIR3, V2R
Dilution WB~~1:1000
FC~~1:25
Target/Specificity This AVPR2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 343-377 amino acids from the C-terminal region of human AVPR2.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAVPR2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AVPR2
Synonyms ADHR, DIR, DIR3, V2R
Function Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Involved in renal water reabsorption.
Cellular Location Cell membrane; Multi-pass membrane protein
Tissue Location Kidney.
Research Areas
Citations (0)
citation

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Background

Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Involved in renal water reabsorption.

References

Seibold A.,et al.Am. J. Hum. Genet. 51:1078-1083(1992).
Birnbaumer M.,et al.Nature 357:333-335(1992).
Wildin R.S.,et al.Am. J. Hum. Genet. 55:266-277(1994).
Fay M.J.,et al.Peptides 17:477-481(1996).
North W.G.,et al.Cancer Res. 58:1866-1871(1998).

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$ 370.00
Cat# AW5433-U100
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