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Mouse Klf4 Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC |
|---|---|
| Primary Accession | Q60793 |
| Reactivity | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | M=52 KDa |
| Isotype | Rabbit IgG |
| Antigen Source | HUMAN |
| Gene ID | 16600 |
|---|---|
| Antigen Region | 321-354 aa |
| Other Names | Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, Klf4, Ezf, Gklf, Zie |
| Dilution | WB~~1:1000 FC~~1:25 |
| Target/Specificity | This Mouse Klf4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 321-354 amino acids from the Central region of mouse Klf4. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mouse Klf4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Klf4 |
|---|---|
| Synonyms | Ezf, Gklf, Zie |
| Function | Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence (PubMed:10431239, PubMed:10556311, PubMed:15358627, PubMed:16954384, PubMed:17060454, PubMed:19816951, PubMed:20071344, PubMed:29593216). Binds to the promoter region of its own gene and can activate its own transcription (PubMed:10431239, PubMed:10556311, PubMed:15358627, PubMed:16954384, PubMed:17060454, PubMed:19816951, PubMed:20071344, PubMed:29593216). Regulates the expression of key transcription factors during embryonic development (PubMed:10431239, PubMed:10556311, PubMed:15358627, PubMed:16954384, PubMed:17060454, PubMed:19816951, PubMed:20071344, PubMed:29593216). Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation (PubMed:10431239, PubMed:10556311, PubMed:15358627, PubMed:16954384, PubMed:17060454, PubMed:19816951, PubMed:20071344, PubMed:29593216). Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription (By similarity). |
| Cellular Location | Nucleus. Cytoplasm |
| Tissue Location | Highest expression in the colon. Lower levels in testis, lung and small intestine |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription (By similarity).
References
Shields J.M.,et al.J. Biol. Chem. 271:20009-20017(1996).
Garrett-Sinha L.A.,et al.J. Biol. Chem. 271:31384-31390(1996).
Mahatan C.S.,et al.Nucleic Acids Res. 27:4562-4569(1999).
Chen Z.-Y.,et al.Exp. Cell Res. 281:19-27(2002).
Carninci P.,et al.Science 309:1559-1563(2005).
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