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EIF3H Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P |
|---|---|
| Primary Accession | O15372 |
| Other Accession | Q6P9U8, Q91WK2, Q5ZLE6, Q56JZ5, Q5PR67, NP_003747.1 |
| Reactivity | Human, Rat |
| Predicted | Zebrafish, Bovine, Chicken, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | H=40;M=40;R=40 KDa |
| Isotype | Rabbit IgG |
| Antigen Source | HUMAN |
| Gene ID | 8667 |
|---|---|
| Antigen Region | 70-99 aa |
| Other Names | Eukaryotic translation initiation factor 3 subunit H {ECO:0000255|HAMAP-Rule:MF_03007}, eIF3h {ECO:0000255|HAMAP-Rule:MF_03007}, Eukaryotic translation initiation factor 3 subunit 3 {ECO:0000255|HAMAP-Rule:MF_03007}, eIF-3-gamma, eIF3 p40 subunit {ECO:0000255|HAMAP-Rule:MF_03007}, EIF3H {ECO:0000255|HAMAP-Rule:MF_03007} |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Target/Specificity | This EIF3H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-99 amino acids from the N-terminal region of human EIF3H. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | EIF3H Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | EIF3H {ECO:0000255|HAMAP-Rule:MF_03007} |
|---|---|
| Function | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl- tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). |
| Cellular Location | Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03007}. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
References
Kupfer, S.S., et al. Gastroenterology 139(5):1677-1685(2010)
Hawken, S.J., et al. Hum. Genet. 128(1):89-101(2010)
Cappuzzo, F., et al. J Thorac Oncol 4(4):472-478(2009)
Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(47):18139-18144(2008)
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