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PLOD1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC |
|---|---|
| Primary Accession | Q02809 |
| Other Accession | Q9R0E2, NP_000293.2 |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | H=84,88;M=84;R=84 KDa |
| Isotype | Rabbit IgG |
| Antigen Source | HUMAN |
| Gene ID | 5351 |
|---|---|
| Antigen Region | 66-94 aa |
| Other Names | Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1, Lysyl hydroxylase 1, LH1, PLOD1, LLH, PLOD |
| Dilution | WB~~1:1000 FC~~1:25 |
| Target/Specificity | This PLOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-94 amino acids from the N-terminal region of human PLOD1. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PLOD1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PLOD1 |
|---|---|
| Synonyms | LLH, PLOD |
| Function | Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils (By similarity). Forms hydroxylysine residues in -Xaa-Lys- Gly- sequences in collagens (PubMed:10686424, PubMed:15854030, PubMed:8621606). These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links (Probable). |
| Cellular Location | Rough endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity.
References
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :
Huang, Q.Y., et al. Bone 44(5):984-988(2009)
Yamada, Y., et al. Int. J. Mol. Med. 19(5):791-801(2007)
Tasker, P.N., et al. Osteoporos Int 17(7):1078-1085(2006)
Giunta, C., et al. Mol. Genet. Metab. 86 (1-2), 269-276 (2005) :
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