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STOM Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - STOM Antibody (N-term) AW5370-U100
    All lanes : Anti-STOM Antibody (N-term) at 1/1000 dilution Lane 1: human lung lysates Lane 2: Hela whole cell lysates Lane 3: HepG2 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 32 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P27105
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=32,13 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 2040
Antigen Region 2-36 aa
Other Names Erythrocyte band 7 integral membrane protein, Protein 72b, Stomatin, STOM, BND7, EPB72
Dilution WB~~1:1000
Target/Specificity This STOM antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2-36 amino acids from the N-terminal region of human STOM.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSTOM Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name STOM (HGNC:3383)
Function Regulates ion channel activity and transmembrane ion transport. Regulates ASIC2 and ASIC3 channel activity.
Cellular Location Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell membrane; Lipid-anchor; Cytoplasmic side. Membrane raft. Melanosome. Cytoplasmic vesicle {ECO:0000250|UniProtKB:P54116}. Note=Localizes to juxtanuclear structure probably derived from the Golgi apparatus (PubMed:9243190) Colocalizes with cortical actin microfilaments at small plasma membrane protrusions (PubMed:9243190). Associates with alpha-granular lipid rafts (PubMed:12130500). Translocates from the alpha-granular lipid rafts to the cell membrane on thrombin activation and selectively enriched in released microvesicles (PubMed:12130500). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545).
Tissue Location Detected in erythrocytes (at protein level). Widely expressed.
Citations (0)
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Background

Thought to regulate cation conductance. May regulate ASIC2 and ASIC3 gating (By similarity).

References

Hiebl-Dirschmied C.M.,et al.Biochim. Biophys. Acta 1090:123-124(1991).
Stewart G.W.,et al.Blood 79:1593-1601(1992).
Unfried I.,et al.Genomics 30:521-528(1995).
Gallagher P.G.,et al.J. Biol. Chem. 270:26358-26363(1995).
Halleck A.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

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$ 370.00
Cat# AW5370-U100
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