CD19 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
---|---|
Primary Accession | P15391 |
Reactivity | Human |
Host | Rabbit |
Clonality | polyclonal |
Calculated MW | H=61 KDa |
Isotype | Rabbit IgG |
Antigen Source | HUMAN |
Gene ID | 930 |
---|---|
Antigen Region | 517-551 aa |
Other Names | B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12, CD19, CD19 |
Dilution | WB~~1:1000 |
Target/Specificity | This CD19 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 517-551 amino acids from the C-terminal region of human CD19. |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | CD19 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CD19 |
---|---|
Function | Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes (PubMed:29523808). Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed:1373518, PubMed:16672701, PubMed:2463100). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed:12387743, PubMed:16672701, PubMed:9317126, PubMed:9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed:9317126). Required for normal differentiation of B-1 cells (By similarity). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:1373518, PubMed:2463100). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:12387743, PubMed:16672701, PubMed:9317126). |
Cellular Location | Cell membrane; Single-pass type I membrane protein. Membrane raft {ECO:0000250|UniProtKB:P25918}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P25918} |
Tissue Location | Detected on marginal zone and germinal center B cells in lymph nodes (PubMed:2463100). Detected on blood B cells (at protein level) (PubMed:16672701, PubMed:2463100) |
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Provided below are standard protocols that you may find useful for product applications.
Background
Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
References
Stamenkovic I.,et al.J. Exp. Med. 168:1205-1210(1988).
Tedder T.F.,et al.J. Immunol. 143:712-717(1989).
Kozmik Z.,et al.Mol. Cell. Biol. 12:2662-2672(1992).
Zhou L.J.,et al.Immunogenetics 35:102-111(1992).
Kuroki K.,et al.Genes Immun. 3:S21-S30(2002).
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