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WDR82 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - WDR82 Antibody (N-term) AW5310-U100
    Fluorescent image of Hela cells stained with WDR82 Antibody (N-term)(Cat#AW5310). AW5310 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
    detail
  • WB - WDR82 Antibody (N-term) AW5310-U100
    Western blot analysis of lysates from NCCIT,SH-SY5Y cell line (from left to right), using WDR82 Antibody (N-term)(Cat. #AW5310). AW5310 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IF, WB
Primary Accession Q6UXN9
Other Accession Q8BFQ4
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=35;M=35;Z=35 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 80335
Antigen Region 12~46 aa
Other Names WD repeat-containing protein 82, Protein TMEM113, Swd2, WDR82, TMEM113, WDR82A
Dilution IF~~1:25
WB~~1:1000
Target/Specificity This WDR82 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 12~46 amino acids from the N-terminal region of human WDR82.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWDR82 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WDR82 {ECO:0000303|PubMed:17998332, ECO:0000312|HGNC:HGNC:28826}
Function Regulatory component of the SET1/COMPASS complex implicated in the tethering of this complex to transcriptional start sites of active genes (PubMed:17998332, PubMed:18838538, PubMed:20516061). Facilitates histone H3 'Lys-4' methylation (H3K4me) via recruitment of the SETD1A or SETD1B to the 'Ser-5' phosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A) (PubMed:17998332, PubMed:18838538). Component of PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase (PubMed:20516061). Together with ZC3H4, but independently of the SET1 complex, part of a transcription termination checkpoint that promotes transcription termination of long non-coding RNAs (lncRNAs) (PubMed:33767452, PubMed:33913806). The transcription termination checkpoint is activated by the inefficiently spliced first exon of lncRNAs and promotes transcription termination of lncRNAs and their subsequent degradation by the exosome (PubMed:33767452).
Cellular Location Nucleus. Chromosome {ECO:0000250|UniProtKB:Q8BFQ4}. Note=Associates with chromatin (PubMed:20516061). Recruited at sites of high RNA polymerase II occupancy (By similarity). {ECO:0000250|UniProtKB:Q8BFQ4, ECO:0000269|PubMed:20516061}
Citations (0)
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Background

Regulatory component of the SET1 complex implicated in the tethering of this complex to transcriptional start sites of active genes. Facilitates histone H3 'Lys-4' methylation via recruitment of the SETD1A or SETD1B to the 'Ser-5' phosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). Component of PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.

References

Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Lee J.-H.,et al.J. Biol. Chem. 280:41725-41731(2005).
Higa L.A.,et al.Nat. Cell Biol. 8:1277-1283(2006).

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$ 370.00
Cat# AW5310-U100
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