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ALDH6A1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • IF - ALDH6A1 Antibody AW5291-U100
    Fluorescent image of MCF-7 cells stained with ALDH6A1 Antibody (Cat#AW5291). AW5291 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
    detail
  • WB - ALDH6A1 Antibody AW5291-U100
    Western blot analysis of lysates from HepG2,T47D cell line (from left to right), using ALDH6A1 Antibody(Cat. #AW5291). AW5291 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
    detail
  • IHC-P - ALDH6A1 Antibody AW5291-U100
    Immunohistochemical analysis of paraffin-embedded H.colon section using ALDH6A1 Antibody(Cat#AW5291). AW5291 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
    detail
  • IHC-P - ALDH6A1 Antibody AW5291-U100
    Immunohistochemical analysis of paraffin-embedded H.liver section using ALDH6A1 Antibody(Cat#AW5291). AW5291 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
    detail
Fluorescent image of MCF-7 cells stained with ALDH6A1 Antibody (Cat#AW5291). AW5291 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, IF, WB
Primary Accession Q02252
Reactivity Human
Predicted Mouse
Host Mouse
Clonality Monoclonal
Calculated MW H=58;M=58 KDa
Isotype IgG1,κ
Antigen Source HUMAN
Additional Information
Gene ID 4329
Antigen Region 104-523 aa
Other Names ALDH6A1; MMSDH; Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial; Aldehyde dehydrogenase family 6 member A1
Dilution IF~~1:25
WB~~1:1000
IHC-P~~1:25
Target/Specificity This ALDH6A1 antibody is generated from mouse immunized with ALDH6A1 recombinant protein.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALDH6A1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALDH6A1 (HGNC:7179)
Function Malonate and methylmalonate semialdehyde dehydrogenase involved in the catabolism of valine, thymine, and compounds catabolized by way of beta-alanine, including uracil and cytidine.
Cellular Location Mitochondrion.
Research Areas
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$ 385.00
Cat# AW5291-U100
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