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COL9A1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - COL9A1 Antibody (Center) AW5258-U100
    Western blot analysis of lysates from MCF-7 cell line and mouse eyeball tissue (from left to right), using COL9A1 Antibody (Center)(Cat. #AW5258). AW5258 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P20849
Other Accession Q05722
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=92,64;M=92,65 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 1297
Antigen Region 428-456 aa
Other Names COL9A1; Collagen alpha-1(IX) chain
Dilution WB~~ 1:1000
Target/Specificity This COL9A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 428-456 amino acids from the Central region of human COL9A1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCOL9A1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name COL9A1
Function Structural component of hyaline cartilage and vitreous of the eye.
Cellular Location Secreted, extracellular space, extracellular matrix
Citations (0)
citation

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Background

COL9A1 is one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules, a heterotrimeric molecule,and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in the COL9A1 gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects.

References

Fresquet,M., J. Biol. Chem. 282 (48), 34634-34643 (2007)
Liu,L.Y., Yi Chuan 29 (4), 427-432 (2007)
Van Camp,G., Am. J. Hum. Genet. 79 (3), 449-457 (2006)
Sivakumaran,T.A., J. Assoc. Res. Otolaryngol. 7 (2), 160-172 (2006)

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$ 370.00
Cat# AW5258-U100
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