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MDH1 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| FC, IHC-P, WB |
|---|---|
| Primary Accession | P40925 |
| Other Accession | O88989, P11708, P14152, Q5ZME2, Q3T145 |
| Reactivity | Human |
| Predicted | Bovine, Chicken, Mouse, Pig, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | H=36,27,39;M=37;Rat=36 KDa |
| Isotype | Rabbit IgG |
| Antigen Source | HUMAN |
| Gene ID | 4190 |
|---|---|
| Antigen Region | 286-314 aa |
| Other Names | MDH1; MDHA; Malate dehydrogenase, cytoplasmic; Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50 |
| Target/Specificity | This MDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-314 amino acids from the C-terminal region of human MDH1. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MDH1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MDH1 {ECO:0000303|PubMed:34012073, ECO:0000312|HGNC:HGNC:6970} |
|---|---|
| Function | Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH (PubMed:2449162, PubMed:3052244). Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation (PubMed:31538237). Catalyzes the reduction of 2-oxoglutarate to 2- hydroxyglutarate, leading to elevated reactive oxygen species (ROS) (PubMed:34012073). |
| Cellular Location | Cytoplasm, cytosol. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.
References
Lee,S.M., et.al., Cell Death Differ. 16 (5), 738-748 (2009)
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