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MYB Antibody (Center)

Purified Mouse Monoclonal Antibody (Mab)

     
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  • WB - MYB Antibody (Center) AW5232-U100
    Western blot analysis of lysates from CEM,Jurkat cell line (from left to right), using MYB Antibody (Center)(Cat. #AW5232). AW5232 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
    detail
  • WB - MYB Antibody (Center) AW5232-U100
    All lanes : Anti-MYB Antibody (Center) at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 80 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P10242
Reactivity Human
Host Mouse
Clonality Monoclonal
Calculated MW H=72 KDa
Isotype IgG1,κ
Antigen Source HUMAN
Additional Information
Gene ID 4602
Antigen Region 1-185 aa
Other Names Transcriptional activator Myb, Proto-oncogene c-Myb, MYB
Dilution WB~~1:2000
Target/Specificity This MYB antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-185 amino acids from the Central region of human MYB.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMYB Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MYB
Function Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625, ECO:0000269|PubMed:19646965}
Research Areas
Citations (0)
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Background

Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.

References

Majello B.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:9636-9640(1986).
Westin E.H.,et al.Oncogene 5:1117-1124(1990).
Close J.P.,et al.BMC Genomics 5:33-33(2004).
Westin E.H.,et al.Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases.
Gaillard C.,et al.Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.

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$ 380.00
Cat# AW5232-U100
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