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NDUFS7 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - NDUFS7 Antibody (Center) AW5221-U100
    Western blot analysis of lysates from Hela cell line,mouse heart,mouse cerebellum tissue lysate (from left to right), using NDUFS7 Antibody (Center)(Cat. #AW5221). AW5221 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession O75251
Other Accession Q9DC70, P42026
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW H=24;M=25 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 374291
Antigen Region 119-146 aa
Other Names NDUFS7;NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial; NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial; Complex I-20kD; NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial; NADH-ubiquinone oxidoreductase 20 kDa subunit; NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial; PSST subunit
Dilution WB~~1:1000
Target/Specificity This NDUFS7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-146 amino acids from the Central region of human NDUFS7.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNDUFS7 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NDUFS7
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:17275378). Essential for the catalytic activity of complex I (PubMed:17275378).
Cellular Location Mitochondrion inner membrane; Peripheral membrane protein {ECO:0000250|UniProtKB:P42026}; Matrix side {ECO:0000250|UniProtKB:P42026}
Research Areas
Citations (0)
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Background

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).

References

Hyslop S.J., et al. Genomics 37:375-380(1996).
Grimwood J., et al. Nature 428:529-535(2004).
Murray J., et al. J. Biol. Chem. 278:13619-13622(2003).
Burkard T.R., et al. BMC Syst. Biol. 5:17-17(2011).
Triepels R.H., et al. Ann. Neurol. 45:787-790(1999).

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$ 370.00
Cat# AW5221-U100
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