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ALDH2 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • IF - ALDH2 Antibody AW5219-U100
    Confocal immunofluorescent analysis of ALDH2 Antibody (Cat#AW5219) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - ALDH2 Antibody AW5219-U100
    Western blot analysis of lysates from NCI-H292,A549,HepG2 cell line (from left to right), using ALDH2 Antibody(Cat. #AW5219). AW5219 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
    detail
  • IHC-P - ALDH2 Antibody AW5219-U100
    ALDH2 Monoclonal Antibody (Cat. #AW5219) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ALDH2 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - ALDH2 Antibody AW5219-U100
    ALDH2 Monoclonal Antibody (Cat. #AW5219) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
    detail
Confocal immunofluorescent analysis of ALDH2 Antibody (Cat#AW5219) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, IF, IHC-P, WB
Primary Accession P05091
Other Accession NP_000681
Reactivity Human
Predicted Mouse, Rat
Host Mouse
Clonality Monoclonal
Calculated MW H=56;M=57;Rat=56 KDa
Isotype IgG1
Antigen Source HUMAN
Additional Information
Gene ID 217
Antigen Region 262-537 aa
Other Names ALDH2; ALDM; Aldehyde dehydrogenase, mitochondrial; ALDH class 2; ALDH-E2; ALDHI
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Target/Specificity This ALDH2 Monoclonal antibody is generated from mouse immunized with ALDH2 recombinant protein.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALDH2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALDH2
Synonyms ALDM
Function Required for clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage.
Cellular Location Mitochondrion matrix.
Research Areas
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$ 385.00
Cat# AW5219-U100
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