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TUFM Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - TUFM Antibody (N-term) AW5218-U100
    Fluorescent image of Hela cells stained with TUFM Antibody (N-term)(Cat#AW5218). AW5218 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
    detail
  • WB - TUFM Antibody (N-term) AW5218-U100
    Western blot analysis of lysates from HepG2,Hela,A431,mouse NIH/3T3 cell line,rat brain tissue lysate(from left to right), using TUFM Antibody (N-term)(Cat. #AW5218). AW5218 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
    detail
  • WB - TUFM Antibody (N-term) AW5218-U100
    All lanes : Anti-TUFM Antibody (N-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: Mouse brain tissue lysate Lane 6: NIH/3T3 whole cell lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IF, WB
Primary Accession P49411
Other Accession P85834, Q8BFR5, P49410
Reactivity Human, Mouse, Rat
Predicted Bovine
Host Rabbit
Clonality polyclonal
Calculated MW H=50;M=50,47;Rat=50 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 7284
Antigen Region 112-147 aa
Other Names Elongation factor Tu, mitochondrial, EF-Tu, P43, TUFM
Dilution IF~~1:25
WB~~1:2000
Target/Specificity This TUFM antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 112-147 amino acids from the N-terminal region of human TUFM.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTUFM Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TUFM
Function Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Also plays a role in the regulation of autophagy and innate immunity. Recruits ATG5-ATG12 and NLRX1 at mitochondria and serves as a checkpoint of the RIGI-MAVS pathway. In turn, inhibits RLR-mediated type I interferon while promoting autophagy.
Cellular Location Mitochondrion.
Research Areas
Citations (0)
citation

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Background

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

References

Woriax V.L.,et al.Biochim. Biophys. Acta 1264:347-356(1995).
Wells J.,et al.FEBS Lett. 358:119-125(1995).
Ling M.,et al.Gene 197:325-336(1997).
Martin J.,et al.Nature 432:988-994(2004).
Dunn M.J.,et al.Submitted (MAR-1996) to UniProtKB.

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$ 370.00
Cat# AW5218-U100
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