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GSS Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, IF |
|---|---|
| Primary Accession | P48637 |
| Other Accession | P46413, P51855, Q8HXX5, Q5EAC2 |
| Reactivity | Human |
| Predicted | Bovine, Monkey, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | H=52;M=52;Rat=52 KDa |
| Isotype | Rabbit IgG |
| Antigen Source | HUMAN |
| Gene ID | 2937 |
|---|---|
| Antigen Region | 372-400 aa |
| Other Names | GSS; Glutathione synthetase; Glutathione synthase |
| Dilution | IF~~1:25 WB~~1:1000 IHC-P~~1:25 |
| Target/Specificity | This GSS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 372-400 amino acids from the C-terminal region of human GSS. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GSS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GSS (HGNC:4624) |
|---|---|
| Function | Catalyzes the production of glutathione from gamma- glutamylcysteine and glycine in an ATP-dependent manner (PubMed:7646467, PubMed:9215686). Glutathione (gamma- glutamylcysteinylglycine, GSH) is the most abundant intracellular thiol in living aerobic cells and is required for numerous processes including the protection of cells against oxidative damage, amino acid transport, the detoxification of foreign compounds, the maintenance of protein sulfhydryl groups in a reduced state and acts as a cofactor for a number of enzymes (PubMed:10369661). Participates in ophthalmate biosynthesis in hepatocytes (By similarity). |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. GSS functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione.
References
Starr,J.M., et.al., Mech. Ageing Dev. 129 (12), 745-751 (2008)
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Anonymous
2016-08-24 01:15:32
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Species tested
Human,Mouse,Rat
Application tested
WB
Brief protocol
1. Block with 3% skim milk for 1 hour at room temperature.
2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃
3. Wash 5*5 min with TBST.
4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature.
5. Wash 5*5 min with TBST.
6. Incubate with ECL substrates and expose |
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