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TAF10 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - TAF10 Antibody (Center) AW5214-U100
    Western blot analysis of lysates from NCI-H292,HepG2 cell line (from left to right), using TAF10 Antibody (Center)(Cat. #AW5214). AW5214 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q12962
Other Accession Q8K0H5, NP_006275.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=22;M=22 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 6881
Antigen Region 121-150 aa
Other Names TAF10; TAF2A; TAF2H; TAFII30; Transcription initiation factor TFIID subunit 10; STAF28; Transcription initiation factor TFIID 30 kDa subunit
Dilution WB~~1:1000
Target/Specificity This TAF10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-150 amino acids from the Central region of human TAF10.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTAF10 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TAF10
Synonyms TAF2A, TAF2H, TAFII30
Function The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed:33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:33795473). TAF10 is also component of the PCAF histone acetylase complex, the TATA-binding protein-free TAF complex (TFTC) and the STAGA transcription coactivator-HAT complex (PubMed:10373431, PubMed:11564863, PubMed:12601814, PubMed:18206972, PubMed:9885574). May regulate cyclin E expression (By similarity).
Cellular Location Nucleus
Citations (0)
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Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the small subunits of TFIID that is associated with a subset of TFIID complexes. Studies with human and mammalian cells have shown that this subunit is required for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and may also be required for certain cellular differentiation programs.

References

Egloff, S., et al. J. Biol. Chem. 285(27):20564-20569(2010)
Ma, Y., et al. J. Biol. Chem. 285(13):9813-9822(2010)
Zhao, Y., et al. Mol. Cell 29(1):92-101(2008)
Hao, H., et al. Oncogene 26(57):7872-7884(2007)
Couture, J.F., et al. Nat. Struct. Mol. Biol. 13(2):140-146(2006)

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$ 370.00
Cat# AW5214-U100
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