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BLMH Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - BLMH Antibody (Center) AW5205-U100
    Fluorescent image of Hela cells stained with BLMH Antibody (Center)(Cat#AW5205). AW5205 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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  • WB - BLMH Antibody (Center) AW5205-U100
    BLMH Antibody (Center) (Cat. #AW5205) western blot analysis in CEM,HL-60 cell line lysates (35ug/lane).This demonstrates the BLMH antibody detected the BLMH protein (arrow).
    detail
  • WB - BLMH Antibody (Center) AW5205-U100
    Western blot analysis of lysates from K562 cell line,mouse stomach tissue lysate(from left to right), using BLMH Antibody (Center)(Cat. #AW5205). AW5205 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession Q13867
Other Accession P70645, P13019, Q8R016
Reactivity Human, Mouse
Predicted Rabbit, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=53;M=53;Rat=52 KDa
Isotype Rabbit IgG
Antigen Source Human
Additional Information
Gene ID 642
Antigen Region 212-242 aa
Other Names BLMH;Bleomycin hydrolase
Dilution IF~~1:25
WB~~1:1000
Target/Specificity This BLMH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-242 amino acids from the Central region of human BLMH.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBLMH Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BLMH
Function The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B- aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.
Cellular Location Cytoplasm. Cytoplasmic granule. Note=Co-localizes with NUDT12 in the cytoplasmic granules.
Research Areas
Citations (0)
citation

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Background

The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity (By similarity).

References

Barrow I.K.-P., et al. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
Ferrando A.A., et al. Cancer Res. 56:1746-1750(1996).
Broemme D., et al. Biochemistry 35:6706-6714(1996).
Kalnine N., et al. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Ota T., et al. Nat. Genet. 36:40-45(2004).

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$ 370.00
Cat# AW5205-U100
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