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NRAS Antibody (N-term)

Mouse Monoclonal Antibody (Mab)

     
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  • WB - NRAS Antibody (N-term) AW5189-U100
    Western blot analysis of lysates from rat C6,A431,MCF-7 cell line (from left to right), using NRAS Antibody (N-term)(Cat. #AW5189). AW5189 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P01111
Other Accession Q04970, Q2MJK3, P08556, Q5F352
Reactivity Human
Predicted Chicken, Mouse, Pig, Rat
Host Mouse
Clonality Monoclonal
Calculated MW H=21 KDa
Isotype IgG1
Antigen Source Human
Additional Information
Gene ID 4893
Antigen Region 70-101 aa
Other Names GTPase NRas; Transforming protein N-Ras;NRAS; HRAS1
Dilution WB~~1:1000
Target/Specificity This NRAS antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 70-101 amino acids from the N-terminal region of human NRAS.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNRAS Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NRAS
Synonyms HRAS1
Function Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor Note=Shuttles between the plasma membrane and the Golgi apparatus
Research Areas
Citations (0)
citation

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Background

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

References

Hall A. et al. Nucleic Acids Res. 13:5255-5268(1985).

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$ 380.00
Cat# AW5189-U100
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