Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
COVID19
>   home   >   Products   >   Primary Antibodies   >   IDH1 Antibody (Center)   

IDH1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
crown
Products labeled with a "crown" meet aggressive quality standards. Learn more.
  • IF - IDH1 Antibody (Center) AW5173-U100
    Confocal immunofluorescent analysis of IDH1 Antibody (Center)(Cat#AW5173) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - IDH1 Antibody (Center) AW5173-U100
    Western blot analysis of lysates from HepG2,MCF-7 cell line and rat liver tissue lysate(from left to right), using IDH1 Antibody (Center)(Cat. #AW5173). AW5173 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
    detail
  • IHC-P - IDH1 Antibody (Center) AW5173-U100
    Immunohistochemical analysis of paraffin-embedded H. prostate section using IDH1 Antibody (Center)(Cat#AW5173). AW5173 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
    detail
  • IHC-P - IDH1 Antibody (Center) AW5173-U100
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDH1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - IDH1 Antibody (Center) AW5173-U100
    IDH1 Antibody (Center) (Cat. #AW5173) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, IF, WB
Primary Accession O75874
Other Accession P41562, O88844, Q9XSG3, Q6XUZ5
Reactivity Human, Rat
Predicted Bovine, Mouse, Sheep
Host Rabbit
Clonality Polyclonal
Calculated MW H=47;M=47;Rat=47 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 3417
Antigen Region 116-143 aa
Other Names IDH1; PICD; Isocitrate dehydrogenase [NADP] cytoplasmic; Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Target/Specificity This IDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-143 amino acids from the Central region of human IDH1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIDH1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IDH1
Synonyms PICD
Function Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed:10521434, PubMed:19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed:10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).
Cellular Location Cytoplasm, cytosol. Peroxisome
Citations (0)
citation

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abcepta antibody to
info@abcepta.com, and receive a free "I Love Antibodies" mug.

Background

IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

References

Geisbrecht B.V., Gould S.J.J. Biol. Chem. 274:30527-30533(1999)
Xu X., Zhao J., Xu Z.J. Biol. Chem. 279:33946-33957(2004)
Bleeker F.E., Lamba S.Hum. Mutat. 30:7-11(2009)

FeedBack
Abcepta welcomes feedback from its customers.

If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abcepta.com.

$ 370.00
Cat# AW5173-U100
Size:
Quantity:
Availability: In Stock
Bulk Size

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaCentral AmericaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSouth KoreaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamWorldwideOthers
Abcepta, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms & Conditions
"