PGK1 Antibody (Center S320)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application ![]()
| IHC-P, WB |
---|---|
Primary Accession | P00558 |
Other Accession | Q60HD8, Q5J7W1 |
Reactivity | Human, Mouse |
Predicted | Monkey |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | H=45;M=45;Rat=45 KDa |
Isotype | Rabbit IgG |
Antigen Source | HUMAN |
Gene ID | 5230 |
---|---|
Antigen Region | 305-334 aa |
Other Names | PGK1; PGKA; Phosphoglycerate kinase 1; Cell migration-inducing gene 10 protein; Primer recognition protein 2 |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Target/Specificity | This PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-334 amino acids from the Central region of human PGK1. |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PGK1 Antibody (Center S320) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PGK1 |
---|---|
Synonyms | PGKA |
Function | Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3- diphosphoglycerate to 3-phosphoglycerate (PubMed:30323285, PubMed:7391028). Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines (PubMed:18463139). In addition to its role as a glycolytic enzyme, it seems that PGK1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). Acts as a protein kinase when localized to the mitochondrion where it phosphorylates pyruvate dehydrogenase kinase PDK1 to inhibit pyruvate dehydrogenase complex activity and suppress the formation of acetyl- coenzyme A from pyruvate, and consequently inhibit oxidative phosphorylation and promote glycolysis (PubMed:26942675, PubMed:36849569). May play a role in sperm motility (PubMed:26677959). |
Cellular Location | Cytoplasm, cytosol. Mitochondrion matrix. Note=Hypoxic conditions promote mitochondrial targeting (PubMed:26942675). Targeted to the mitochondrion following phosphorylation by MAPK1/ERK2, cis-trans isomerization by PIN1, and binding to mitochondrial circRNA mcPGK1 (PubMed:36849569). |
Tissue Location | Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men |

If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.