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Phospho-SHP2(Y546) Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, DB |
|---|---|
| Primary Accession | Q06124 |
| Other Accession | P41499, P35235, Q90687 |
| Reactivity | Human, Mouse |
| Predicted | Chicken, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | H=68 KDa |
| Isotype | Rabbit IgG |
| Antigen Source | HUMAN |
| Gene ID | 5781 |
|---|---|
| Antigen Region | 521-551 aa |
| Other Names | PTPN11; PTP2C; SHPTP2; Tyrosine-protein phosphatase non-receptor type 11; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; SH-PTP2; SH-PTP3 |
| Dilution | WB~~1:1000 DB~~1:500 |
| Target/Specificity | This SHP2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y546 of human SHP2. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-SHP2(Y546) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PTPN11 |
|---|---|
| Synonyms | PTP2C, SHPTP2 |
| Function | Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus (PubMed:10655584, PubMed:14739280, PubMed:18559669, PubMed:18829466, PubMed:26742426, PubMed:28074573). Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity (PubMed:18559669). Dephosphorylates CDC73 (PubMed:26742426). Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification (By similarity). Dephosphorylates tyrosine-phosphorylated NEDD9/CAS-L (PubMed:19275884). |
| Cellular Location | Cytoplasm. Nucleus |
| Tissue Location | Widely expressed, with highest levels in heart, brain, and skeletal muscle. |
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Provided below are standard protocols that you may find useful for product applications.
Background
SHP2 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration.
References
Nystrom,A.M., et.al., Eur J Med Genet (2010) In press
Carver,K.C., et.al., J. Biol. Chem. 285 (11), 8003-8012 (2010)
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