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Phospho-HIST1H3B3(S10) Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - Phospho-HIST1H3B3(S10) Antibody AW5153-U100
    Western blot analysis of lysate from Hela cell line, using Phospho-H3-pS10 Antibody (Cat. #AW5153). AW5153 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysate at 20ug.
    detail
  • WB - Phospho-HIST1H3B3(S10) Antibody AW5153-U100
    Western blot analysis of lysate from Hela cell line, using Phospho-H3-pS10 Antibody (Cat. #AW5153). AW5153 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysate at 20ug.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P68431
Reactivity Human
Predicted Mouse, Rat, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW H=15,M=15,Rat=15 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968
Antigen Region 8-35 aa
Other Names HIST1H3A; H3FA; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
Dilution WB~~1:500
Target/Specificity This HIST1H3B3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human HIST1H3B3.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-HIST1H3B3(S10) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name H3C1 (HGNC:4766)
Synonyms H3FA, HIST1H3A
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Research Areas
Citations (0)
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Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

References

Lusic, M., et al., EMBO J. 22(24):6550-6561 (2003).
Deng, L., et al., Virology 289(2):312-326 (2001).
Deng, L., et al., Virology 277(2):278-295 (2000).
El Kharroubi, A., et al., Mol. Cell. Biol. 18(5):2535-2544 (1998).
Albig, W., et al., Hum. Genet. 101(3):284-294 (1997).

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$ 380.00
Cat# AW5153-U100
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