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MDH2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - MDH2 Antibody (Center) AW5150-U100
    Western blot analysis of lysates from A431,Hela,U-87 MG,NIH/3T3 cell line (from left to right), using MDH2 Antibody (Center)(Cat. #AW5150). AW5150 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
    detail
  • IHC-P - MDH2 Antibody (Center) AW5150-U100
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with MDH2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - MDH2 Antibody (Center) AW5150-U100
    Flow cytometric analysis of K562 cells using MDH2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, FC, WB
Primary Accession P40926
Other Accession P04636, P00346, P08249, Q4R568, O02640, Q32LG3, P22133
Reactivity Human, Mouse
Predicted Yeast, Bovine, C.Elegans, Monkey, Pig, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=36;M=36;Rat=36 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 4191
Antigen Region 86-115 aa
Other Names MDH2; Malate dehydrogenase, mitochondrial
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Target/Specificity This MDH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-115 amino acids from the Central region of human MDH2.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMDH2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MDH2
Cellular Location Mitochondrion matrix {ECO:0000250|UniProtKB:P04636}
Research Areas
Citations (0)
citation

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Background

MDH2 is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.

References

Rzem,R.,et.al., J. Inherit. Metab. Dis. 30 (5), 681-689 (2007)

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$ 370.00
Cat# AW5150-U100
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