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PDCL Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - PDCL Antibody (N-term) AW5123-U100
    Western blot analysis of lysates from Y79,Hela,Jurkat cell line (from left to right), using PDCL Antibody (N-term)(Cat. #AW5123). AW5123 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
    detail
  • IHC-P - PDCL Antibody (N-term) AW5123-U100
    Immunohistochemical analysis of paraffin-embedded H.kidney section using PDCL Antibody (N-term)(Cat#AW5123). AW5123 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB
Primary Accession Q13371
Other Accession Q63737, Q9DBX2, Q2HJA9
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=34;M=34;Rat=34 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 5082
Antigen Region 1-30 aa
Other Names Phosducin-like protein, PHLP, PDCL, PHLOP1, PhLP1
Dilution WB~~1:1000
IHC-P~~1:25
Target/Specificity This PDCL antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PDCL.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPDCL Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PDCL
Synonyms PHLOP1, PhLP1
Function Acts as a positive regulator of hedgehog signaling and regulates ciliary function. [Isoform 2]: Acts as a negative regulator of heterotrimeric G proteins assembly by trapping the preloaded G beta subunits inside the CCT chaperonin.
Cellular Location Cell projection, cilium {ECO:0000250|UniProtKB:Q9DBX2}
Citations (0)
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Background

Isoform 1: Functions as a co-chaperone for CCT in the assembly of heterotrimeric G protein complexes, facilitates the assembly of both Gbeta-Ggamma and RGS-Gbeta5 heterodimers.

References

Lazarov M.E.,et al.Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Humphray S.J.,et al.Nature 429:369-374(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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$ 370.00
Cat# AW5123-U100
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