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PITPNA Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - PITPNA Antibody (C-term) AW5110-U100
    Western blot analysis of lysates from Hela cell line,mouse brain,mouse cerebellum tissue,mouse NIH/3T3,rat PC-12 cell line,rat brain,rat cerebellum tissue,U-251 MG cell line (from left to right), using PITPNA Antibody (C-term)(Cat. #AW5110). AW5110 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
    detail
  • WB - PITPNA Antibody (C-term) AW5110-U100
    All lanes : Anti-PITPNA Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: U-251 MG whole cell lysate Lane 5: mouse brain lysate Lane 6: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 32 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - PITPNA Antibody (C-term) AW5110-U100
    All lanes : Anti-PITPNA Antibody (C-term) at 1:2000 dilution Lane 1: NIH/3T3 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: MOLT-4 whole cell lysate Lane 5: U-251 MG whole cell lysate Lane 6: mouse brain lysate Lane 7: rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 32 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - PITPNA Antibody (C-term) AW5110-U100
    Immunohistochemical analysis of paraffin-embedded H. skin section using PITPNA Antibody (C-term)(Cat#AW5110). AW5110 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q00169
Other Accession P16446, P48738, P53810, Q2HJ54
Reactivity Human, Mouse, Rat
Predicted Bovine, Rabbit
Host Rabbit
Clonality Polyclonal
Calculated MW H=32;M=32;Rat=32 KDa
Isotype Rabbit IgG
Antigen Source Human
Additional Information
Gene ID 5306
Antigen Region 243-270 aa
Other Names Phosphatidylinositol transfer protein alpha isoform;PITPN; Phosphatidylinositol transfer protein alpha isoform
Dilution WB~~1:2000
IHC-P~~1:25
Target/Specificity This PITPNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-270 amino acids from the C-terminal region of human PITPNA.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPITPNA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PITPNA
Synonyms PITPN
Function Catalyzes the transfer of phosphatidylinositol (PI) and phosphatidylcholine (PC) between membranes (PubMed:10531358, PubMed:14962392, PubMed:15522822, PubMed:18636990, PubMed:22822086). Shows a preference for PI and PC containing shorter saturated or monosaturated acyl chains at the sn-1 and sn-2 positions (PubMed:15522822, PubMed:22822086). Preference order for PC is C16:1 > C16:0 > C18:1 > C18:0 > C20:4 and for PI is C16:1 > C16:0 > C18:1 > C18:0 > C20:4 > C20:3 (PubMed:22822086).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:P53810}. Nucleus {ECO:0000250|UniProtKB:P53810}
Citations (0)
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Background

Catalyzes the transfer of PtdIns and phosphatidylcholine between membranes.

References

Dickeson S.K., et al. Gene 142:301-305(1994).
Tanaka S., et al. Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases.
Lubec G., et al. Submitted (MAR-2007) to UniProtKB.
Rikova K., et al. Cell 131:1190-1203(2007).
Choudhary C., et al. Science 325:834-840(2009).

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$ 370.00
Cat# AW5110-U100
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