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HSF2 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - HSF2 Antibody (Center) AW5101-U100
    Western blot analysis of lysates from A549,Hela cell line (from left to right), using HSF2 Antibody (Center)(Cat. #AW5101). AW5101 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q03933
Other Accession NP_004497.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=60,58;M=60,58 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 3298
Antigen Region 216-245 aa
Other Names HSF2; HSTF2; Heat shock factor protein 2; Heat shock transcription factor 2
Dilution WB~~1:1000
Target/Specificity This HSF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-245 amino acids from the Central region of human HSF2.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSF2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSF2
Synonyms HSTF2
Function DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth and moves to the nucleus upon activation
Citations (0)
citation

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Background

HSF2, as well as the related gene HSF1, encodes a protein that binds specifically to the heat-shock element and has homology to HSFs of other species. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Although the names HSF1 and HSF2 were chosen for historical reasons, these peptides should be referred to as heat-shock transcription factors.

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Xing, H., et al. Cell Stress Chaperones 15(3):301-308(2010)
Sandqvist, A., et al. Mol. Biol. Cell 20(5):1340-1347(2009)
Tateishi, Y., et al. J. Biol. Chem. 284(4):2435-2447(2009)
Vieira, A.R., et al. Genet. Med. 10(9):668-674(2008)

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$ 370.00
Cat# AW5101-U100
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