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CRTAP Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - CRTAP Antibody (N-term) AW5097-U100
    Fluorescent image of Hela cells stained with CRTAP Antibody (N-term)(Cat#AW5097). AW5097 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
    detail
  • WB - CRTAP Antibody (N-term) AW5097-U100
    Western blot analysis of lysates from HT1080,ZR-75-1 cell line (from left to right), using CRTAP Antibody (N-term)(Cat. #AW5097). AW5097 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IF, WB
Primary Accession O75718
Other Accession NP_006362.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=47;M=46 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 10491
Antigen Region 31-59 aa
Other Names CRTAP; CASP; Cartilage-associated protein
Dilution IF~~1:25
WB~~1:1000
Target/Specificity This CRTAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-59 amino acids from the N-terminal region of human CRTAP.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCRTAP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CRTAP
Synonyms CASP
Function Necessary for efficient 3-hydroxylation of fibrillar collagen prolyl residues.
Cellular Location Secreted, extracellular space, extracellular matrix
Tissue Location Found in articular chondrocytes. Expressed in a variety of tissues
Citations (0)
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Background

The protein encoded by this gene is similar to the chicken and mouse CRTAP genes. The encoded protein is a scaffolding protein that may influence the activity of at least one member of the cytohesin/ARNO family in response to specific cellular stimuli. Defects in this gene are associated with osteogenesis imperfecta, a connective tissue disorder characterized by bone fragility and low bone mass.

References

Morello, R., et al. Curr Osteoporos Rep 8(2):77-83(2010)
Li, G.H., et al. Osteoporos Int 21(6):1009-1020(2010)
Fratzl-Zelman, N., et al. Bone 46(3):820-826(2010)
Chang, W., et al. Hum. Mol. Genet. 19(2):223-234(2010)
Marini, J.C., et al. Cell Tissue Res. 339(1):59-70(2010)

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$ 370.00
Cat# AW5097-U100
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