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ENO1 Antibody

Mouse Monoclonal Antibody (Mab)

     
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  • WB - ENO1 Antibody AW5060-U100
    Western blot analysis of lysates from MCF-7 cell line,mouse stomach,mouse liver tissue lysate,Hela,HepG2,NIH/3T3,A431 cell line (from left to right), using ENO1 Antibody(Cat. #AW5060). AW5060 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
    detail
  • IHC-P - ENO1 Antibody AW5060-U100
    Immunohistochemical analysis of paraffin-embedded H. kideny section using ENO1 Antibody(Cat#AW5060). AW5060 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB
Primary Accession P06733
Reactivity Human, Mouse
Predicted Rat
Host Mouse
Clonality Monoclonal
Calculated MW H=47;M=47;Rat=47 KDa
Isotype IgG2b,k
Antigen Source Human
Additional Information
Gene ID 2023
Antigen Region 1-415 aa
Other Names ENO1;ENO1L1; MBPB1; MPB1; Alpha-enolase; Alpha-enolase; 2-phospho-D-glycerate hydro-lyase; Alpha-enolase; C-myc promoter-binding protein; Alpha-enolase; Enolase 1; Alpha-enolase; MBP-1; Alpha-enolase; MPB-1; Alpha-enolase; Non-neural enolase; Alpha-enolase; Phosphopyruvate hydratase; Alpha-enolase; Plasminogen-binding protein
Dilution WB~~1:1000
IHC-P~~1:25
Target/Specificity Purified His-tagged ENO1 protein was used to produced this monoclonal antibody.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsENO1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ENO1
Synonyms ENO1L1, MBPB1, MPB1
Function Glycolytic enzyme the catalyzes the conversion of 2- phosphoglycerate to phosphoenolpyruvate (PubMed:1369209, PubMed:29775581). In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed:10802057, PubMed:12666133, PubMed:2005901, PubMed:29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed:12666133). Stimulates immunoglobulin production (PubMed:1369209).
Cellular Location Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line
Tissue Location The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons
Citations (0)
citation

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Background

Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

References

Giallongo A., et al. Proc. Natl. Acad. Sci. U.S.A. 83:6741-6745(1986).
Giallongo A., et al. Eur. J. Biochem. 190:567-573(1990).
Ray R., et al. Mol. Cell. Biol. 11:2154-2161(1991).
Walter M., et al. J. Autoimmun. 8:931-945(1995).
Kalnine N., et al. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.

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$ 380.00
Cat# AW5060-U100
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