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TBB5 Antibody
Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, IF, WB |
|---|---|
| Primary Accession | P07437 |
| Other Accession | P09244, Q91575, P69897, Q767L7, P99024, P69893, Q2KJD0 |
| Reactivity | Human, Mouse, Rat |
| Predicted | Bovine, Hamster, Pig, Xenopus, Chicken |
| Host | Mouse |
| Clonality | Monoclonal |
| Calculated MW | H=50;M=50;Rat=50 KDa |
| Isotype | IgG2b,κ |
| Antigen Source | HUMAN |
| Gene ID | 203068 |
|---|---|
| Antigen Region | 1-219 aa |
| Other Names | TUBB; TUBB5; Tubulin beta chain; Tubulin beta-5 chain |
| Dilution | IF~~1:25 WB~~1:1000 IHC-P~~1:10~50 |
| Target/Specificity | TBB5 recombinant protein is used to produce this monoclonal antibody. |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | TBB5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TUBB |
|---|---|
| Synonyms | TUBB5 |
| Function | Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. |
| Cellular Location | Cytoplasm, cytoskeleton |
| Tissue Location | Ubiquitously expressed with highest levels in spleen, thymus and immature brain. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
References
An approach based on a genome-wide association study reveals candidate loci for narcolepsy. Shimada M, et al. Hum Genet, 2010 Oct. PMID 20677014. High-density SNP screening of the major histocompatibility complex in systemic lupus erythematosus demonstrates strong evidence for independent susceptibility regions. Barcellos LF, et al. PLoS Genet, 2009 Oct. PMID 19851445. Proteome analysis of schizophrenia patients Wernicke's area reveals an energy metabolism dysregulation. Martins-de-Souza D, et al. BMC Psychiatry, 2009 Apr 30. PMID 19405953. Identification of interaction partners for individual SH3 domains of Fas ligand associated members of the PCH protein family in T lymphocytes. Linkermann A, et al. Biochim Biophys Acta, 2009 Feb. PMID 19041431. Alterations in oligodendrocyte proteins, calcium homeostasis and new potential markers in schizophrenia anterior temporal lobe are revealed by shotgun proteome analysis. Martins-de-Souza D, et al. J Neural Transm, 2009 Mar. PMID 19034380.
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If you have any additional inquiries please email technical services at tech@abcepta.com.
Anonymous
2016-08-24 01:15:32
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Species tested
Human,Mouse
Application tested
WB
Brief protocol
1. Block with 3% skim milk for 1 hour at room temperature.
2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃
3. Wash 5*5 min with TBST.
4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature.
5. Wash 5*5 min with TBST.
6. Incubate with ECL substrates and expose |
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