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Mouse Tfap2a Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - Mouse Tfap2a Antibody (Center) AW5049-U100
    Western blot analysis of lysates from mouse thymus, rat testis tissue and mouse NIH/3T3 cell line (from left to right), using MOUSE Tfap2a Antibody (Center)(Cat. #AW5049). AW5049 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P34056
Other Accession P58197, A1A4R9, Q9N0N3
Reactivity Mouse, Rat
Predicted Bovine, Sheep
Host Rabbit
Clonality Polyclonal
Calculated MW H=48,M=48,47,51,Rat=48 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 21418
Antigen Region 134-166 aa
Other Names Transcription factor AP-2-alpha, AP2-alpha, AP-2 transcription factor, Activating enhancer-binding protein 2-alpha, Activator protein 2, AP-2, Tfap2a, Ap2tf, Tcfap2a
Dilution WB~~1:1000
Target/Specificity This mouse Tfap2a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 134-166 amino acids from the Central region of mouse Tfap2a.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Tfap2a Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Tfap2a
Synonyms Ap2tf, Tcfap2a
Function Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.
Cellular Location Nucleus.
Citations (0)
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Background

Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.

References

Moser M.,et al.Nucleic Acids Res. 21:4844-4844(1993).
Meier P.,et al.Dev. Biol. 169:1-14(1995).
Carninci P.,et al.Science 309:1559-1563(2005).
Mitchell P.J.,et al.Genes Dev. 5:105-119(1991).
Yahata T.,et al.Genomics 80:601-613(2002).

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$ 370.00
Cat# AW5049-U100
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