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LYZ Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - LYZ Antibody (C-term) AW5031-U100
    Western blot analysis of lysates from HepG2, THP-1, WiDr cell line and human spleen tissue lysate(from left to right), using LYZ Antibody (C-term)(Cat. #AW5031). AW5031 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
    detail
  • WB - LYZ Antibody (C-term) AW5031-U100
    All lanes : Anti-LYZ Antibody (C-term) at 1:2000 dilution Lane 1: WiDr whole cell lysate Lane 2: THP-1 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: Human spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - LYZ Antibody (C-term) AW5031-U100
    All lanes : Anti-LYZ Antibody (C-term) at 1:2000 dilution Lane 1: THP-1 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Human spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - LYZ Antibody (C-term) AW5031-U100
    Immunohistochemical analysis of paraffin-embedded M. small intestine section using LYZ Antibody (C-term)(Cat#AW5031). AW5031 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
    detail
  • IHC-P - LYZ Antibody (C-term) AW5031-U100
    Immunohistochemical analysis of paraffin-embedded R. small intestine section using LYZ Antibody (C-term)(Cat#AW5031). AW5031 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
    detail
  • IHC-P - LYZ Antibody (C-term) AW5031-U100
    Immunohistochemical analysis of paraffin-embedded H. small intestine section using LYZ Antibody (C-term)(Cat#AW5031). AW5031 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
    detail
  • IHC-P - LYZ Antibody (C-term) AW5031-U100
    AW5031 staining LYZ in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • IHC - LYZ Antibody (C-term) AW5031-U100
    Immunohistochemical analysis of paraffin-embedded Human spleen section using Pink1(Cat#AW5031). AW5031 was diluted at 1:1000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB
Primary Accession P61626
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=17 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 4069
Antigen Region 119-154 aa
Other Names Lysozyme C, 4-beta-N-acetylmuramidase C, LYZ, LZM
Dilution WB~~1:2000
IHC-P~~1:25
IHC~~1:1000
Target/Specificity This LYZ antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 119-154 amino acids from the C-terminal region of human LYZ.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLYZ Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LYZ
Synonyms LZM
Function Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
Cellular Location Secreted.
Research Areas
Citations (0)
citation

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Background

Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte- macrophage system and enhance the activity of immunoagents.

References

Castanon M.J.,et al.Gene 66:223-234(1988).
Chung L.P.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:6227-6231(1988).
Yoshimura K.,et al.Biochem. Biophys. Res. Commun. 150:794-801(1988).
Peters C.W.B.,et al.Eur. J. Biochem. 182:507-516(1989).
Huang B.,et al.Sheng Wu Hua Hsueh Tsa Chih 9:269-273(1993).

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$ 370.00
Cat# AW5031-U100
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