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RPL6 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - RPL6 Antibody (N-term) AW5024-U100
    Fluorescent image of HeLa cells stained with RPL6 Antibody (N-term)(Cat#AW5024). AW5024 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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  • WB - RPL6 Antibody (N-term) AW5024-U100
    Western blot analysis of lysates from Hela, NCI-H1299 cell line (from left to right),using RPL6 Antibody (N-term)(Cat. #AW5024). AW5024 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
    detail
  • IHC-P - RPL6 Antibody (N-term) AW5024-U100
    Immunohistochemical analysis of paraffin-embedded H. gastric carcinoma section using RPL6 Antibody (N-term)(Cat#NA). NA was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
    detail
  • FC - RPL6 Antibody (N-term) AW5024-U100
    Flow cytometric analysis of Jurkat cells using RPL6 Antibody (N-term)(green, Cat#AW5024) compared to an isotype control of rabbit IgG(blue). AW5024 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, IF, FC, WB
Primary Accession Q02878
Reactivity Human
Host Rabbit
Clonality polyclonal
Calculated MW H=33 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 6128
Antigen Region 2-35 aa
Other Names 60S ribosomal protein L6, Neoplasm-related protein C140, Tax-responsive enhancer element-binding protein 107, TaxREB107, RPL6, TXREB1
Dilution IF~~1:25
WB~~1:1000
IHC-P~~1:25
FC~~1:25
Target/Specificity This RPL6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2-35amino acids from the N-terminal region of human RPL6.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPL6 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPL6
Synonyms TXREB1
Function Component of the large ribosomal subunit (PubMed:12962325, PubMed:23636399, PubMed:25901680, PubMed:25957688, PubMed:32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:12962325, PubMed:23636399, PubMed:25901680, PubMed:25957688, PubMed:32669547).
Cellular Location Cytoplasm, cytosol. Cytoplasm Rough endoplasmic reticulum {ECO:0000250|UniProtKB:Q2YGT9} Note=Detected on cytosolic polysomes (PubMed:25957688). Detected in ribosomes that are associated with the rough endoplasmic reticulum (By similarity). {ECO:0000250|UniProtKB:Q2YGT9, ECO:0000269|PubMed:25957688}
Citations (0)
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Background

Specifically binds to domain C of the Tax-responsive enhancer element in the long terminal repeat of HTLV-I.

References

Zaman G.J.R.,et al.Nucleic Acids Res. 21:1673-1673(1993).
Morita T.,et al.AIDS Res. Hum. Retroviruses 9:115-121(1993).
Kenmochi N.,et al.J. Hum. Genet. 45:290-293(2000).
Ohta K.,et al.J. Mol. Endocrinol. 12:85-92(1994).
Odintsova T.I.,et al.J. Protein Chem. 22:249-258(2003).

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$ 370.00
Cat# AW5024-U100
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